Overcoming Polymyxin Resistance in <i>Klebsiella pneumoniae</i> with <i>Ocotea</i> Essential Oils: Insights from In Vitro and In Vivo Analyses.

ABSTRACT: Antimicrobial resistance, particularly in carbapenem-polymyxin-resistant Klebsiella pneumoniae (CPR-Kp), is a major challenge associated with severe infections. In this study, we assessed the chemical composition and antimicrobial properties of essential oils (EOs) from Ocotea diospyrifolia (OdEO) and Ocotea velloziana (OvEO) against CPR-Kp. The EOs were extracted from the leaves via hydrodistillation and analyzed using gas chromatography-mass spectrometry. The antimicrobial activities of the EOs, alone and along with polymyxin B (OdEO-PMB and OvEO-PMB), were assessed through checkerboard assays, survival curves, and biofilm inhibition. Cell membrane permeability, reactive oxygen species levels, and scanning electron microscopy (SEM) were used to investig

SUBMITTER: Faccin ID 

PROVIDER: S-EPMC12771118 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature