Loading of STING Agonist into Lipid Nanoparticles Boosts Dendritic Cell Activation.

ABSTRACT: Activation of the STING pathway is crucial for antitumor immunity, but targeting the STING receptor therapeutically remains a challenge due to inefficient intracellular delivery of its agonists. Here, we explore the potential of FDA-approved lipid nanoparticles (LNPs), used in Spikevax COVID-19 vaccine, to increase the uptake of a cGAMP analog (cG^sA^sMP) to dendritic cells (DCs) and induce cell activation. These LNPs are highly reproducible, effectively encapsulate cG^sA^sMP, and are easily scalable. We show that cG^sA^sMP LNPs are efficiently internalized by DCs in vitro, and the cargo is released into the cytoplasm, which strongly enhances STING activation and DC maturation. Furthermore, we introduce an in vitro m

SUBMITTER: Ribeiro AR 

PROVIDER: S-EPMC12771213 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature