Formatotrophic <i>Komagataella phaffii</i> expressing recombinant xylanase via metabolic engineering.

ABSTRACT: Formate utilization as a sustainable carbon source for microbial production of high-value chemicals and heterologous proteins presents considerable safety and environmental benefits over conventional feedstocks. As a low-cost, CO₂-derived compound, formate serves as a non-flammable and non-toxic alternative to methanol for induction of recombinant expression in Komagataella phaffii. However, since native K. phaffii only utilizes formate as an energy source rather than a carbon substrate for biomass synthesis, we engineered a synthetic peroxisomal formate assimilation pathway by introducing heterologous acetyl-CoA synthetase (ACS) and acetaldehyde dehydrogenase (ACDH), thereby enabling formatotrophic growth with formate as the sole carbon source. This chassis was fu

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC12771328 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature