Three-Dimensional Tumor Spheroids of Pancreatic and Colorectal Cancer Cell Lines for High-Throughput Screening of Drug Candidates.

ABSTRACT: Over the past decade, three-dimensional (3D) cancer models have emerged as crucial tools in high-throughput screening (HTS), providing a more reliable bridge between in vitro and in vivo drug testing. These models demonstrate higher predictive accuracy compared to traditional two-dimensional (2D) cultures, although the lack of reproducibility and standardization is the main challenge for their translation into quantitative disease modeling and therapeutic response. Therefore, optimization and standardization of 3D spheroid cultivation protocols for HTS for various cancer cell types remain pertinent. This study establishes optimized protocols for cultivating four cancer cell lines (HCT116, SW620, PANC-1, and BxPc-3) as 3D spheroids for drug screening applications. Through systematic paramet

SUBMITTER: Trofimov M 

PROVIDER: S-EPMC12771431 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature