Development of a Lateral Flow Immunoassay Strips for Rapid Antigen Detection of Dabie bandavirus.

ABSTRACT: This study reports the development of a point-of-care (POC) serological assay for the detection of the Dabie bandavirus (DBV) infection. Specifically, this assay is based on the principle of lateral flow immunoassay (LFIA) strips. Two monoclonal antibodies against DBV were generated. They showed an optimal pairing. One mAb, named NP-10, was immobilized on the strip surface as the capture antibody. The other mAb, named NP-20, was labeled with colloidal gold nanoparticles (AuNPs) and used as the detector antibody. A series of parameters of this method were optimized during the AuNPs-mAb immune complex conjugating process, including the different pairing of antigens and antibodies, pH value, and the amount of coupled antibody. The whole detection process was completed within 15 min using 70 μ

SUBMITTER: Huang C 

PROVIDER: S-EPMC12771437 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature