Characterisation of a Novel Jumbo Lytic Aeromonas dhakensis Bacteriophage P19 and Its Endolysin.

ABSTRACT: Aeromonas dhakensis is an emerging multidrug-resistant (MDR) bacterial pathogen that presents significant threats to public health and aquaculture. This study reports the isolation and comprehensive characterisation of jumbo bacteriophage P19. It is a lytic phage with a long, contractile tail and is placed in the genus Ceceduovirus of the family Straboviridae. P19 possesses a large genome typical of jumbo phages, which likely contributes to its enhanced replication efficiency and adaptability. P19 exhibited strong lytic activity with a high efficiency of plating (EOP) against multiple A. dhakensis strains, a burst size of approximately 50 plaque-forming units (PFU) per cell, and a latent period of 30 min. It remained highly stable across pH 6-10 and temperatures up to 60°C. Genomic analysi

SUBMITTER: Ansari F 

PROVIDER: S-EPMC12771599 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature