Utility of an <i>in vitro</i> lymphatics on-chip model for rank ordering subcutaneous absorption of monoclonal antibodies.

ABSTRACT: The lymphatic vasculature plays a key role in the subcutaneous absorption of macromolecules (>16 kDa). Recent trends toward subcutaneous delivery of macromolecular therapeutics have brought awareness to the need for preclinical estimation of subcutaneous bioavailability prior to first-in-human studies. In vitro tools offer a low-cost means to inform molecule design and formulation and mitigate costly mistakes of under- or overestimation of therapeutic dose and exposure in clinical studies. Building on a previous engineered on-chip lymphatics platform, the utility of an in vitro model to rank therapeutic proteins based on lymphatic absorption was investigated. Lymphatics grown under a combination of interstitial flow and growth factor supplementation on-chip demonstrated in

SUBMITTER: Ledo AM 

PROVIDER: S-EPMC12771666 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature