Differential effects of fetal bovine serum and human platelet lysate on mesenchymal stromal cell-mediated support of hematopoietic stem/progenitor cells: a functional and transcriptomic analysis.

ABSTRACT:

Background

Umbilical cord blood (UCB) is a valuable source of hematopoietic stem and progenitor cells (HSPC) for transplantation. However, limited cell numbers require ex vivo expansion for effective treatment of adult patients. Mesenchymal stromal cell (MSC)-based co-cultures offer a supportive environment for HSPC expansion and a model to study niche interactions. Human platelet lysate (hPL) provides a xeno-free alternative to fetal bovine serum (FBS) for MSC culture, but its effect on MSC hematopoietic support remains unclear. This study investigates transcriptomic and functional changes induced by hPL-based culture and their impact on HSPC regulation.

Methods

MSC from three bone marrow donors were expanded using hPL- or FBS-supplemented media under three regimens: contin

SUBMITTER: Carreira MC 

PROVIDER: S-EPMC12771711 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature