Single-cell transcriptome analysis defines novel molecular subtypes and reveals therapeutic implications of T/myeloid mixed-phenotype acute leukemia.

ABSTRACT:

Background

T/myeloid mixed-phenotype acute leukemia (T/My MPAL) is a malignant disease characterized by co-expression of lymphoid and myeloid features. The lack of molecular classification of T/My MPAL results in highly heterogeneity in treatment responses and clinical outcomes. Identifying molecular subtypes and developing subtype-specific treatment strategies are crucial for improving prognosis and enabling personalized therapies.

Methods

We constructed a single-cell transcriptomic landscape of T/My MPAL, acute myeloid leukemia (AML), T-cell acute lymphoid leukemia (T-ALL), and normal donors by analyzing nearly 275,000 cells. Malignant cells were identified using lineage-specific markers and healthy reference datasets. By comparing the whole transcriptomic profiles of T/My

SUBMITTER: Huang B 

PROVIDER: S-EPMC12771788 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature