Calcium-dependent serine-threonine phosphatase and autophagy inactivation mediated by Baicalein attenuates prion protein-mediated neuronal cell damage.

ABSTRACT:

Background

Prion diseases are a group of incurable and fatal neurodegenerative disorders characterized by neuronal cell death. Calcineurin and autophagy induce neurotoxicity by prion proteins, which may have therapeutic benefits by inhibiting calcineurin and autophagy. Baicalein is known to have neuroprotective effects against calcium-dependent cell death in aging and neurodegenerative diseases.

Methods

Western blot analysis was used to detect the expression levels of autophagy flux pathway. Transmission electron microscopy (TEM) was used to detect autophagy flux. A calcineurin cellular activity assay kit was used to examine calcineurin activity. Fluo-4 was used to measure calcium inside living cells by confocal microscope.

Results

In the present study, it was confirm

SUBMITTER: Hong JM 

PROVIDER: S-EPMC12771857 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature