Multi-omics analysis reveals the association between intratumoral bacteria and somatic mutational signatures in oral squamous cell carcinoma.

ABSTRACT:

Background

Oral squamous cell carcinoma (OSCC) poses significant challenges with its obscure etiology and poor prognosis. Intratumoral bacteria exert a significant influence on the progression of OSCC. Nonetheless, the precise composition and role of the intratumoral bacteria in OSCC patients remain obscure and the association between the intratumoral bacteria and somatic mutational signatures in OSCC patients has not been defined.

Methods

Paraffin-embedded tumors and adjacent normal tissues were collected retrospectively from OSCC patients. A five-region 16S rRNA gene sequencing (5R 16S rRNA-seq) approach (targeting V2, V3, V5, V6, and V8) was employed to characterize the intratumoral bacterial composition of OSCC patients, while whole-exome sequencing (WES) was utilized to

SUBMITTER: Dong Y 

PROVIDER: S-EPMC12771913 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature