A simple and standardized method supports efficient derivation of clinical-grade human embryonic stem cells under feeder- and xeno-free conditions.

ABSTRACT:

Background

Human embryonic stem cells (hESCs), as naturally pluripotent stem cells, constitute a pivotal cell source for cell replacement therapies. Yet, the generation of clinically compliant hESC lines under feeder-free and xeno-free conditions remains inefficient. Moreover, the derivation of hESCs from clinically surplus and discarded low-quality embryos using the standardized, translation-ready culture systems has not been reported.

Methods

By optimizing culture conditions and inner cell mass (ICM) isolation method, we developed a method that significantly improves the derivation efficiency of hESC lines from clinically surplus and discarded frozen-thawed embryos under feeder- and xeno-free conditions. The derivation protocol is operationally simple and easily standardiz

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC12772070 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature