Molecular analysis of group B protective surface protein, a new cell surface protective antigen of group B streptococci.

ABSTRACT: Group B streptococci (GBS) express various surface antigens designated c, R, and X antigens. A new R-like surface protein from Streptococcus agalactiae strain Compton R has been identified by using a polyclonal antiserum raised against the R protein fraction of this strain to screen a lambda Zap library. DNA sequence analysis of positive clones allowed the prediction of the primary structure of a 105-kDa protein designated BPS protein (group B protective surface protein) that exhibited typical features of streptococcal surface proteins such as a signal sequence and a membrane anchor region but did not show significant similarity with other known sequences. Immunogold electron microscopy using a BPS-specific antiserum confirmed the surface location of BPS protein on S. agalactiae strain Com

SUBMITTER: Erdogan S 

PROVIDER: S-EPMC127721 | biostudies-literature | 2002 Feb

REPOSITORIES: biostudies-literature