A High-Resolution Subcellular Map of Proteins in Cells with Motile Cilia.

ABSTRACT: Motile cilia are complex structures regulated by thousands of genes, essential for various physiological functions like respiration and reproduction. Their dysfunction can result in severe conditions like primary ciliary dyskinesia (PCD), highlighting the need for a deeper molecular understanding of their specific ciliary compartments. Interestingly, ciliated cells harbor multiple proteins with limited evidence on biological function, as defined by Functional Evidence (FE) scores, a grading system developed by the Human Proteome Project (HPP). Building upon the stringent antibody validation pipeline of the Human Protein Atlas (HPA) project, we developed a high-throughput workflow that combines a novel multiplex immunohistochemistry protocol with image analysis to investigate protein expres

SUBMITTER: Bertilsson F 

PROVIDER: S-EPMC12772120 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature