Blood Matrices and Sample Preparation Influence Blood Marker Discovery.

ABSTRACT: While plasma and serum are widely used in high-throughput proteomics, the impact of different blood matrix types remains underexplored. Routine diagnostics most commonly use serum or Li-heparin plasma, while the proteomics community primarily focuses on advancing analytical depth in EDTA plasma. Here, we systematically investigated the LC-MS/MS proteomic profiles of pooled blood samples from three healthy, voluntary probands including serum (with/without separation gel) and plasma anticoagulated with EDTA, citrate, or Li-heparin. Sample preparation was conducted with the commercially available iST and ENRICH-iST kits, strong-anion exchange (SAX) beads, the TFA-based approach SPEED, and perchloric acid (perCA) precipitation. Mass-spectrometric measurements were performed on a Q Exactive HF-

SUBMITTER: Gronauer TF 

PROVIDER: S-EPMC12772121 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature