Isolation and identification of a genotype F bovine enterovirus in western China.

ABSTRACT: This study successfully isolated a novel bovine enterovirus strain from a bovine fecal sample, which was designated as Sichuan/SQ/20. The isolate showed typical enterovirus morphology under electron microscopy. Phylogenetic analysis showed that this strain exhibits the closest genetic relationship with the HeN-YR91 and JPN/TottoriU-31 strains, and all three belong to the BEV-F1 genosubtype. Subsequently, comprehensive investigations were conducted on the biological characteristics of this virus, both in vivo and in vitro. In vitro characterization revealed that viral replication commenced at 3 h post-infection (hpi) in Madin-Darby bovine kidney cells, reaching peak at 48 hpi with a virus titer of 1 × 10^8.73 TCID₅₀/0.1 mL. Cytopathic effects initia

SUBMITTER: Xu K 

PROVIDER: S-EPMC12772302 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature