<i>Leishmania donovani</i> elongator protein Elp3a plays a crucial role in modulating the parasite response to genotoxic stress.

ABSTRACT: The eukaryotic elongator complex comprises six proteins Elp1-Elp6, with Elp3 being the catalytic subunit. In trypanosomatids, only Elp3 has been identifiable, and interestingly, these organisms have two Elp3 orthologs: Elp3a and Elp3b. This study was undertaken to examine the role of Elp3a in the trypanosomatid Leishmania donovani. The elp3a gene was successfully eliminated, with the insect form as well as mammalian form of the parasite surviving and propagating normally in its absence, signifying its non-essential nature under usual growth conditions. In examining the response of LdElp3a-depleted parasites to genotoxic agents, we found no behavioral differences in response to UV and camptothecin, but the parasites exhibited higher tolerance to methyl methane sulfonate and ch

SUBMITTER: Khanna A 

PROVIDER: S-EPMC12772310 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature