Histidinol dehydrogenase (HisD): a critical regulator of <i>Staphylococcus aureus</i> virulence and a promising target for antivirulence therapy.

ABSTRACT: Staphylococcus aureus is a pathogen responsible for diverse severe infections. The global spread of methicillin-resistant S. aureus (MRSA) necessitates innovative therapeutic approaches beyond traditional antibiotics. S. aureus virulence mechanisms remain a critical concern. Targeting histidinol dehydrogenase (HisD), a key enzyme in histidine biosynthesis, presents a novel anti-virulence strategy. We used the derivative strains of Newman strains (ΔhisD, ΔhisD::pRAB-hisD, and WT::pRAB-hisD) and clinical strains to study the role of HisD in the pathogenicity of S. aureus. HisD inhibition by pixantrone was further evaluated. The absence of hisD significantly reduced hemolytic activity and biofilm formation, accompanied by the down

SUBMITTER: Jiang W 

PROVIDER: S-EPMC12772333 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature