<i>Coxiella burnetii</i> Nine Mile II Δ<i>cbu0533</i> as a suitable laboratory replacement strain.

ABSTRACT: Coxiella burnetii is an intracellular bacterial pathogen that causes the disease Q fever. Both secretion of effector proteins by a type IVB secretion system and expression of lipopolysaccharide (LPS) are critical for C. burnetii virulence. The C. burnetii Nine Mile strain lineage is routinely used in laboratories, with Nine Mile I RSA493 (fully virulent) expressing phase I LPS, Nine Mile Crazy RSA514 (lightly attenuated) expressing intermediate LPS, and NMII RSA439 clone 4 (NMII, attenuated) expressing phase II LPS. NMII was exempted from the U.S. Centers for Disease Control and Prevention Federal Select Agent Program due to a genetic deletion predicted to prevent phase I LPS synthesis; however, recent findings demonstrated that NMII LPS elongation occurs in vivo

SUBMITTER: Pierce KN 

PROVIDER: S-EPMC12772341 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature