Ampicillin promotes the biofilm formation of <i>Shewanella putrefaciens</i> through the c-di-GMP-regulated BpfAGD system.

ABSTRACT: Although many Shewanella strains are naturally resistant to some β-lactam antibiotics, research into the effect of these antibiotics on Shewanella biofilm formation is scarce. Shewanella putrefaciens is not only known as an important seafood spoilage bacterium but can also cause infection in several aquatic animals. In addition, it is a rare opportunistic human pathogen. The present study found that although some antibiotics from cephalosporins, carbapenems, and monobactams repress the biofilm formation of S. putrefaciens CN32, multiple penicillin antibiotics increase its biofilm formation. Further experiments showed that ampicillin can increase intracellular c-di-GMP levels by regulating 16 DGCs/PDEs. This increases biofilm formation of S. putrefaciens C

SUBMITTER: Shi R 

PROVIDER: S-EPMC12772357 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature