Spatio-Temporal Diversity of Calcium Activity in Microglia.

ABSTRACT: Microglia, the brain's innate immune cells, possess complex, highly motile branched processes. These act independently, enabling individual processes to carry out entirely distinct functions in parallel. Intracellular Ca²⁺ signaling is implicated in many of these distinct microglial functions. However, it has been difficult to quantify how such Ca²⁺ activity is compartmentalized in space and time to prevent unwanted cross-talk between signaling pathways. Previous studies have typically relied on manually drawn regions-of-interest (ROIs), which averages fluorescence within predefined compartments and therefore cannot resolve the fine-scale spatio-temporal propagation patterns that may be functionally relevant. To address this, we adopt an unbiased non-ROI-based analyti

SUBMITTER: Horiuchi H 

PROVIDER: S-EPMC12772526 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature