Lamin A/C directs nucleosome-scale chromatin remodeling to define early lineage segregation in mammals.

ABSTRACT: Chromatin organization underlies gene regulation and cell fate specification, yet how nucleosome-scale chromatin structure contributes to lineage segregation during early development remains unknown. Here, we resolved chromatin ultrastructure during the first lineage decision in mouse and human, which forms the pluripotent inner cell mass (ICM) and trophectoderm (TE). To achieve this, we developed dual-tilt chromatin electron tomography (2T-ChromEMT) that allows multiscale visualization of chromatin architecture. Our analysis reveals that TE cells of both species display denser chromatin with nucleosome aggregation at the nuclear periphery. We show upregulation of the nuclear matrix protein Lamin A/C within the TE lineage across mouse, human, and opossum embryos, indicating that its regula

SUBMITTER: Sherrard A 

PROVIDER: S-EPMC12773000 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature