Development of Entebbe Bat Virus Reverse Genetics System and Phenotypic Evaluation of Rescued Virus Reveals Host-Specific Replication Patterns in Mosquitoes.

ABSTRACT: Entebbe bat virus (ENTV) is a bat-associated flavivirus with no known arthropod vector. Research into the biology of this virus, including assessment of the possibility that it may be vector-transmitted, is hindered by a lack of molecular tools and robust genetic systems. Therefore, the complete 3' untranslated region, which was not previously available, was sequenced, and an infectious clone of ENTV was developed to facilitate further investigation of the virus. Virus derived from the clone replicated similarly to the parental virus isolate in various vertebrate cells. Surprisingly, ENTV replicated to high titers in the Aedes aegypti (Ae. aegypti) and Aedes albopictus (Ae. albopictus) mosquito cell lines, but there was no replication or infection in Culex tarsalis cells. In addition, phyl

SUBMITTER: Fujii M 

PROVIDER: S-EPMC12774305 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature