Discovery and validation of ASG1 as a novel determinant of NaCl tolerance in the yeast Saccharomyces cerevisiae through iterative crossing.

ABSTRACT: To dissect the genetic basis of quantitative traits, generation of numerous haploid segregants with diverse genotypes and phenotypes from heterozygous parental strains is a powerful approach. To identify quantitative trait loci (QTLs) associated with NaCl salt tolerance, we employed an iterative crossing strategy using parental strains with contrasting phenotypes. Whole-genome sequencing of selected individual offspring with the most extreme trait value from each generation as well as of the pools of segregants under extreme salt conditions enabled QTL mapping and identification of candidate causative variants. Their effects on phenotypic variation were quantified through a genome-wide screen of generation-dependent reduction of the causative loci and by allele swapping procedure of the pu

SUBMITTER: Zun G 

PROVIDER: S-EPMC12774596 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature