Spatial transcriptomics uncovers lipid metabolic dysregulation driving immune-stroma crosstalk in Sjogren's Disease.

ABSTRACT:

Background

Sjögren's Disease (SjD) is a systemic autoimmune disorder characterised by exocrine gland dysfunction and immune infiltration. However, the spatial cellular architecture and metabolic-immune crosstalk underlying glandular pathology in SjD remain unclear.

Methods

We performed spatial transcriptomics on SjD salivary glands (SGs) and conducted integrative analyses with single-cell RNA sequencing to delineate disease-specific transcriptomic alterations, followed by validation in an independent cohort. Key findings were verified via immunofluorescence, immunohistochemistry, and function assays on cells.

Findings

Spatial clustering revealed distinct perturbations in SjD. We identified a pathogenic CCL2^high fibroblast-ACKR1^high endothelial ce

SUBMITTER: Li S 

PROVIDER: S-EPMC12774687 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature