Biological functional identification of holins from Salmonella pullorum phages and their therapeutic efficacy against pullorum disease in chickens.

ABSTRACT: Salmonella pullorum (S.pullorum) is a significant pathogen that seriously endangers the poultry industry. However, its prevention and control mainly rely on antibiotics, which leads to the wide spread of drug-resistant genes and poses a serious public health risk. Phage lytic proteins have received extensive attention in recent years as potential alternatives to antibiotics. In this study, whole genome sequencing and phylogenetic analysis demonstrated that Salmonella Pullorum phage vB_SpuM_23 is most closely related to Salmonella phages SWJM-02 and SalM SPJ41. Two holin proteins (Holin-1 and Holin-P) were identified from the phage genome. Expression in the Escherichia coli system confirmed the broad-spectrum antibacterial activity of Holin-1, Holin-P, and their conjugate holin-p-holin-1, w

SUBMITTER: Zhao H 

PROVIDER: S-EPMC12774745 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature