Redox investigation of L-A9 peptide and evaluation of its binding with double stranded DNA.

ABSTRACT: L-A9 is one of the important short peptides having biomedical applications in relation to its affinity towards HER2 receptors. The peptide has been synthesised using manual solid phase synthesis protocol and the redox property has been investigated using the voltammetric techniques. The oxidation peak of the peptide has shown strong pH dependency, revealed the 1 e and 2 proton transfer process. Electroanalytical method is developed with limit of detection of 1.17 × 10^-7 M. The modulation of the redox property has been monitored to investigate the binding of the peptide with dsDNA, with binding constant of 1.8 × 10⁵ M^- 1. Spectroscopic measurements of the peptide revealed the pH dependent electronic transition and the binding constant with dsDNA of 1.4 × 10⁴

SUBMITTER: Dwivedi P 

PROVIDER: S-EPMC12774949 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature