SOSHI-seq: a high-throughput screening assay to test the functionality of putative response elements for nuclear hormone receptors.

ABSTRACT: We present a cheap, rapid, and versatile assay called SOSHI-seq (Screening of Self-transcribed Hormone Inducible response elements coupled to sequencing). The protocol is an adaptation of the STARR-seq method, which allows testing the capacity of thousands of synthetic DNA sequences to act as hormone-response elements. Using the nuclear receptors of thyroid hormone as an example, we show that SOSHI-seq is a suitable complement to ChIP-Seq analysis to identify at genome-wide scale the functional response elements occupied by nuclear receptors in chromatin.

SUBMITTER: Wu S 

PROVIDER: S-EPMC12775020 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature