2,8-Dihydroxyadenine disrupts epithelial integrity and alters kidney cell phenotype in vitro.

ABSTRACT: Adenine phosphoribosyltransferase (APRT) deficiency is an autosomal recessive disorder that causes accumulation of 2,8-dihydroxyadenine (DHA) in the urinary tract, leading to kidney stones and chronic kidney disease (CKD). Progression to end-stage kidney disease can occur without timely treatment. The xanthine oxidoreductase inhibitors, allopurinol and febuxostat, block DHA generation and halt or delay stone formation and CKD progression. Some patients do not tolerate xanthine oxidoreductase inhibitor treatment, necessitating new therapeutic approaches. This study aimed to investigate how DHA influences structural and molecular changes in HK-2, HEK293, and MDCK kidney cells. DHA exposure reduced cell viability and impaired migration in all cell lines. Increased expression of the adhesion p

SUBMITTER: Helgudottir HR 

PROVIDER: S-EPMC12775057 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature