Cationic amino acid transporters (CAT) enhance accumulation and susceptibility to the systemic herbicide L-phosphinothricin.

ABSTRACT: The broad-spectrum herbicide L-phosphinothricin (PPT) irreversibly inhibits glutamine synthetase (GS) activity. Here we present cationic amino acid transporters (CAT) as mediators of PPT accumulation and susceptibility. Comparative analysis of Arabidopsis thaliana, Marchantia polymorpha, rice, and Klebsormidium nitens reveals conserved responses to GS inhibition, with absence of efficient PPT uptake underlying resistance in M. polymorpha. Transcriptomic analysis identifies four candidate transporters in A. thaliana which, when overexpressed in M. polymorpha, confer varying levels of sensitivity corresponding with PPT accumulation. AtCAT1 and AtCAT5 confer the greatest sensitivities and are required for glutamic acid uptake and endogenous nitrogen metabolism in A. thaliana, and PPT suscepti

SUBMITTER: Tan GZH 

PROVIDER: S-EPMC12775149 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature