CircEPHB4 binds to YBX1 to upregulate MRPS16 and promotes glioma progression.

ABSTRACT:

Background

Glioma is the most frequently diagnosed brain tumor in adults worldwide which is associated with unfavorable prognosis and survival time. However, the understanding of glioma progression remains limited.

Methods

The cell proliferation in glioma cells were monitored by EdU incorporation and CCK-8 assays. Glioma cell invasion and migration were assessed by Transwell assay. In vivo tumorigenesis were detected by xenograft study with bioluminescence imaging. qRT-PCR, RNA FISH, IHC or western blot were used to detect circEPHB4, MRPS16, YBX1, RBBP6 and other molecules expression. The associations between YBX1 and MRPS16 mRNA, as well as between circEPHB4 and YBX1, were detected by RNA immunoprecipitation (RIP) and RNA pull-down assays. In addition, the ubiquitination of

SUBMITTER: Liao Y 

PROVIDER: S-EPMC12775211 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature