PERP suppresses breast cancer metastasis via the ATF3-HSPA6 signaling pathway.

ABSTRACT: ObjectiveThe role of the tumor suppressor p53 apoptosis effector related to PMP-22 (PERP) in breast cancer metastasis remains unclear. This study investigated PERP's role in metastatic progression, its clinical significance, and the mechanisms underlying its effects.MethodsPERP expression was assessed in breast cancer cell lines, public datasets, and 142 patient samples using real-time quantitative polymerase chain reaction (RT-qPCR), western blotting, and immunohistochemistry. In vitro migration and invasion assays as well as in vivo metastasis model were performed after PERP overexpression or knockdown in MDA-MB-231 and MCF-7 cells. The roles of activating transcription factor 3 (ATF3) and heat shock protein family A member 6 (HSPA6) were evaluated through small-interfering RNA-mediated

SUBMITTER: Chen C 

PROVIDER: S-EPMC12775323 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature