Dissecting Hhip1 Function <i>In Vivo</i> Using a Conditional Knockout Mouse Model.

ABSTRACT: The Hedgehog (Hh) signaling pathway is essential for organ development and tissue homeostasis; its dysregulation is associated with various congenital anomalies and human diseases. Hedgehog-interacting protein 1 (Hhip1) functions as a membrane-bound negative regulator of Hh signaling by directly binding to Hh ligands and limiting their activity in target tissues. While global knockout models have demonstrated the importance of Hhip1 during embryonic development, perinatal lethality has precluded investigations into its postnatal functions. To overcome this limitation, a novel conditional allele of Hhip1 (hereafter referred to as the Hhip1^flox or floxed allele, in which exon 2 is flanked by loxP sites) was generated using CRISPR/Cas9-based gene targeting. Cre

SUBMITTER: Haraguchi R 

PROVIDER: S-EPMC12775407 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature