Dual targeting of AMRC12 and <i>Malassezia globosa</i> disrupts MYC liquid condensates-driven nuclear pore complex biogenesis in neuroblastoma.

ABSTRACT: Rationale: Neuroblastoma (NB) is a predominant extra-cranial malignancy in childhood, while molecular drivers of its progression and effective treatment strategies have yet to be clarified. Methods: RNA sequencing was performed to identify transcriptional regulators and corresponding target genes. To explore the biological effects and underlying mechanisms of these regulators, a comprehensive methodology was utilized, encompassing chromatin immunoprecipitation, dual-luciferase reporter assay, qRT-PCR, western blot, alongside gene over-expression and silencing techniques, co-immunoprecipitation, and mass spectrometry. The MTT assay, soft agar colony formation, Matrigel invasion, and nude mouse xenograft models were applied to assess oncogenic properties. Patient survival was a

SUBMITTER: Hu A 

PROVIDER: S-EPMC12775802 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature