Metabolic and epigenetic abnormalities cause hepatic fibrogenesis in metabolic dysfunction-associated steatohepatitis model mice.

ABSTRACT: The prevalence rates of liver-related metabolic syndrome, metabolic dysfunction-associated steatotic liver disease, and the consequent metabolic dysfunction-associated steatohepatitis (MASH) are increasing worldwide. There is a well-established and reproducible choline-deficient l-amino acid-defined high-fat diet (CDAHFD)-induced MASH model, but the cause of the hepatic fibrogenesis in this model is unclear. We evaluated the phenotypic changes associated with hepatic fibrogenesis in CDAHFD-fed mice using RNA sequencing, GC-MS, and LC-MS-based metabolic analyses. A time-series liver transcriptomic analysis revealed inflammation and activated matrix remodeling within 1 week in the CDAHFD-fed mouse liver. Also, metabolomic analysis revealed increased activity in the pentose phosphate pathway,

SUBMITTER: Miura A 

PROVIDER: S-EPMC12775946 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature