FlashDeconv enables atlas-scale, multi-resolution spatial deconvolution via structure-preserving sketching.

ABSTRACT: Coarsening Visium HD resolution from 8 to 64 μm can flip cell-type co-localization from negative to positive ( r=-0.12→+0.80 ), yet investigators are routinely forced to coarsen because current deconvolution methods cannot scale to million-bin datasets. Here we introduce FlashDeconv, which combines leverage-score importance sampling with sparse spatial regularization to match top-tier Bayesian accuracy while processing 1.6 million bins in 153 seconds on a standard laptop. Systematic multi-resolution analysis of Visium HD mouse intestine reveals a tissue-specific resolution horizon (8-16 μ m)-the scale at which this sign inversion occurs-validated by Xenium ground truth. Below this horizon, FlashDeconv provides the first sequencing-based quantification of Tuft ce

SUBMITTER: Yang C 

PROVIDER: S-EPMC12776147 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature