Proteomic analysis of altered urinary extracellular vesicle levels in breast cancer patients pre- and post-surgery.

ABSTRACT:

Background

Urinary extracellular vesicles (uEVs) have shown potential as non-invasive biomarkers for various cancers. However, research on the relationship between uEVs levels and cargo changes after breast cancer (BC) surgery remains limited. We investigated changes in uEVs levels and proteomes in patients with BC pre- and post-surgery.

Methods

uEVs isolated from patients with BC (n=30) pre- and post-surgery were characterized using western blotting, transmission electron microscopy, nanoparticle tracking analysis, and proteomic analysis. Based on post- versus pre-surgery uEVs levels, patients were classified into increasing (n=8) and decreasing (n=22) trend groups.

Results

Proteomic analysis revealed remarkable patterns of protein expression pre- and post-surgery in

SUBMITTER: Jeanmard N 

PROVIDER: S-EPMC12776214 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature