Demethylzeylasteral alleviates myeloid leukemia through PERK/eIF2α/ATF4/CHOP-mediated cell apoptosis.

ABSTRACT:

Background

Myeloid leukemia, as a malignant tumor of hematologic system, urgently requires discovery of novel therapeutic drugs. Demethylzeylasteral (DML), a natural terpenoid primary extract from Tripterygium, has demonstrated antitumor properties across various cancer types. However, its functions in myeloid leukemia have not been clearly elucidated. This study aimed to explore the effects of DML on the proliferation and apoptosis of myeloid leukemia cells, and elucidate its mechanism.

Methods

Cell viability was detected by Cell Count Kit-8 (CCK-8) method. Post-DML treatment, cell cycle, apoptosis, intensity of unfolded proteins, adenosine triphosphate (ATP), intracellular calcium, and reactive oxygen species (ROS) levels were assessed by flow cytometry. Additionally, tran

SUBMITTER: Li X 

PROVIDER: S-EPMC12776217 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature