HSP90α and KLK6 Co-Regulate Stress-Induced Prostate Cancer Cell Motility.

ABSTRACT:

Background

Prostate cancer (PCa) metastasis is reliant on the activity of proteases, such as matrix metalloproteinase-2 (MMP-2). While increased extracellular heat shock protein 90α (eHSP90α) has been linked to increased MMP-2 activity, this has not been examined in the context of cellular stress.

Methods

We examined stress-induced eHSP90α in human prostate cell lines by immunoblot. Fluorometric gelatin dequenching and zymography assays measured MMP activity. Wound healing and Matrigel drop invasion assays were used to quantify cell motility. HSP90α knockout (KO) cells were established with CRISPR/Cas9. Proteases were profiled with molecular inhibitors and protein arrays and validated by siRNA knockdown, immunoblot, and motility assays.

Results

Stress increased eHSP90

SUBMITTER: O'Neill KL 

PROVIDER: S-EPMC12776274 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature