IncL plasmid-mediated dissemination of OXA-48 β-lactamase and bla _CTX-M-15 gene amplification identified via long-read sequencing in carbapenem-resistant Enterobacterales.

ABSTRACT:

Background

Increasing resistance to broad-spectrum beta-lactams and carbapenems is a significant concern in healthcare settings. This study aimed to determine the prevalence of intestinal carriage of extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Enterobacterales (CRE) in a tertiary care hospital and to evaluate the utility of long-read sequencing for carbapenem resistance surveillance.

Methods

In 2021, stool samples (n = 538) and rectal swabs (n = 256) from hospitalized patients were cultured after enrichment on selective chromogenic medium to detect ESBL and CRE carriage. CRE isolates were characterized by antimicrobial susceptibility testing and whole-genome sequencing.

Results

Among 794 patient samples, 239 (30%) Enterobacter

SUBMITTER: Prasad S 

PROVIDER: S-EPMC12776359 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature