Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.

ABSTRACT: High-speed volumetric imaging of the brain is essential for linking diverse cellular events to tissue-level functions. However, the brain's structural and dynamic heterogeneity-spanning microns to millimeters and milliseconds to hours-requires imaging techniques with tunable spatiotemporal resolution, flexible 3D sampling, and compatibility with targeted perturbations. Here, we present tunable Bessel beam two-photon fluorescence microscopy (tBessel-TPFM), a compact, low-cost, and versatile platform for intravital brain imaging across millimeter scale with subcellular resolution. tBessel-TPFM transforms slow 3D volume scans into fast 2D frame scans via an axially elongated Bessel focus, achieving acquisition rates ~100-fold faster and reduced motion artifacts compared with conventional TPFM

SUBMITTER: Li MJ 

PROVIDER: S-EPMC12776403 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature