Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy.

ABSTRACT: Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-plex imaging strategy that integrates optimized cryo-fixation for antigen preservation, expansion microscopy, and iterative immunofluorescence labeling. Using oblique plane microscopy, we perform three-dimensional super-resolution imaging of 20 biological targets encompassing the full cellular volume of individual mammalian cells.

SUBMITTER: Galecki S 

PROVIDER: S-EPMC12776423 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature