Detergent exchange from lipid nanoparticles into detergent micelles unlocks a tool for biochemical and kinetic characterization of membrane proteins.

ABSTRACT: Bacterial membrane proteins make up ~30% of the prokaryotic genome and play key roles in infection and virulence. Membrane protein chemistry has advanced in recent years, including purification strategies that mimic "native-like" lipid environments, such as lipid nanoparticles, amphipols, and nanodiscs. The use of styrene maleic acid co-polymers to form a lipid nanoparticle has become increasingly common in membrane protein purification, especially for proteins which are not amenable to detergent extraction from the cellular membrane fraction. Yet, for some biochemical and biophysical methods it is preferable to use detergent-solubilized protein. Here we show a general exchange method to transfer membrane proteins from lipid nanoparticles to detergent micelles while retaining protein fold,

SUBMITTER: Koweek RS 

PROVIDER: S-EPMC12776464 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature