Feedback from the Nascent Chain Triggers Ribosomal Frameshifting and Transcript Decay.

ABSTRACT: Though ribosomes have several features that help them maintain their reading frame, these safeguards can be bypassed by RNA structures that promote -1 programmed ribosomal frameshifting (-1PRF). We recently found that conformational transitions in the nascent polypeptide can enhance -1PRF, though it's unclear whether this feedback plays a general role in translational recoding. Here we demonstrate that the translocation of nascent transmembrane domains is sufficient to induce -1PRF during the decoding of slippery heptamers. We identify thousands of motifs that potentially trigger -1PRF along with proteomic identifications of 33 predicted human frameshift products. We also identify thousands of splicing-dependent motifs and demonstrate that the splicing-mediated reconfiguration of transmemb

SUBMITTER: Carmody PJ 

PROVIDER: S-EPMC12776465 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature