Differential fluorescence induction analysis of Streptococcus pneumoniae identifies genes involved in pathogenesis.

ABSTRACT: Differential fluorescence induction (DFI) technology was used to identify promoters of Streptococcus pneumoniae induced under various in vitro and in vivo conditions. A promoter-trap library using green fluorescent protein as the reporter was constructed in S. pneumoniae, and the entire library was screened for clones exhibiting increased gfp expression under the chosen conditions. The in vitro conditions used were chosen to mimic aspects of the in vivo environment encountered by the pathogen once it enters a host: changes in temperature, osmolarity, oxygen, and iron concentration, as well as blood. In addition, the library was used to infect animals in three different models, and clones induced in these environments were identified. Several promoters were identified in multiple screens, a

SUBMITTER: Marra A 

PROVIDER: S-EPMC127766 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature