Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process.

ABSTRACT:

Objective

Conversion of lignocellulosic biomass to renewable biofuels and bio-based chemicals at scale holds great promise for increasing domestic supplies of energy and alternatives to petrochemicals but is contingent upon improvements in biomass deconstruction. Here we report a method to maximize the enzymatic hydrolysis of whole plant samples containing fibrous and non-fibrous carbohydrates in sorghum, a naturally drought-tolerant high-yielding bioenergy feedstock. This process involves the addition of amylases to ionic liquid-pretreated biomass in conjunction with traditionally employed cellulase and hemicellulase cocktails.

Results

We found that amylases are compatible with cholinium-based ionic liquids and act synergistically with other saccharolytic enzymes to deconst

SUBMITTER: Islam MT 

PROVIDER: S-EPMC12777037 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature