Excess prenatal folic acid supplementation alters cortical DNA methylation and gene expression networks.

ABSTRACT:

Introduction

Folate is crucial for various biological processes, and deficiencies during pregnancy are linked to increased risk for neural tube defects and neurodevelopmental disorders. As a proactive measure, folic acid fortification of staple foods has been mandated in many countries, in addition to dietary supplementation recommendations during pregnancy. However, the risks of excess prenatal folic acid supply have yet to be fully understood.

Methods

To better appreciate molecular changes in mouse brain exposed to in utero 5-fold folic acid excess over normal intake, we investigated the transcriptome and methylome for alterations in gene networks.

Results

RNA-seq analysis of cerebral cortex collected at birth, revealed significant expression differences in 6

SUBMITTER: Haghani V 

PROVIDER: S-EPMC12777089 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature