Targeting CXCL8 alleviates intestinal epithelial inflammation by suppressing the JAK-STAT pathway.

ABSTRACT:

Background

Inhibition of CXCL8 has been shown to alleviate small intestinal inflammation and suppress activation of Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. However, the mechanism of the interaction between the two requires further exploration.

Methods

Human small intestinal epithelial cells were stimulated with lipopolysaccharide (LPS) to establish an inflammatory model. The expression of pro-inflammatory factors was detected using qRT-PCR. Cell viability and apoptosis in cells treated with LPS or phosphate buffered saline (PBS) were assessed by CCK-8 assay and flow cytometry, respectively. Western blot was conducted to detect the expression of proteins linked to the JAK-STAT signaling pathway. Then, cell viability, apoptos

SUBMITTER: Wu X 

PROVIDER: S-EPMC12777093 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature