Targeting ubiquitin-specific protease 14 reduces metastatic potential and metabolic activity in cervical cancer via direct modulation of monocarboxylate transporter-4.

ABSTRACT:

Background

Cervical cancer (CC) remains a major health burden in low- and middle-income countries, where HPV vaccination coverage is suboptimal. Ubiquitin-specific peptidase 14 (USP14), a proteasome-associated deubiquitinase, has emerged as a potential driver of tumorigenesis, but its role in CC and impact on tumor metabolism remain poorly defined.

Methods

In-silico analysis of RNA and proteomics data from publicly available datasets was performed to assess the expression and prognostic value of USP14. Expression was validated in CC cell lines (HeLa, SiHa, CaSki) in comparison to non-transformed MCF10A cells. Functional studies involved USP14 knockdown (siRNA) or inhibition (IU1), followed by various cell viability, metastasis and invasion assays. Comparative proteomics was

SUBMITTER: Chauhan R 

PROVIDER: S-EPMC12777198 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature