Deep-freezing and decontamination strategy for a large autologous bone graft with presentation of the osteogenic potential of resident osteoblasts.

ABSTRACT:

Background

Musculoskeletal injuries resulting from high-impact trauma often lead to extensive bone defects that require effective reconstruction to prevent limb amputation. However, contamination of large autologous bone grafts in open fractures remains a major clinical challenge. This study aimed to establish a reliable protocol for decontamination of massive autologous bone graft and to assess the viability and regenerative potential of osteoblasts from frozen bone, including the effects of deep freezing and the cryoprotectant dimethyl sulfoxide (DMSO).

Methods

A 28 cm femoral segment, extruded during a high-energy road traffic accident, was stored at - 80 °C for 15 days before microbiological analysis revealed contamination. The bone was thawed, decontaminated, and succes

SUBMITTER: Zekusic M 

PROVIDER: S-EPMC12777315 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature